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Image Search Results
Journal: Materials Today Bio
Article Title: Synergized photothermal therapy and magnetic field induced hyperthermia via bismuthene for lung cancer combinatorial treatment
doi: 10.1016/j.mtbio.2023.100825
Figure Lengend Snippet: Ex-vivo bismuthene immunocompatibility in PBMCs (a) Impact of bismuthene on PBMC viability. PBMCs were treated with different concentrations (10, 50, 100 and 200 μg/mL) of bismuthene for 24 h. Cell viability was analyzed by flow cytometry using Fixable Viability Dye 780 staining (BD). Ethanol at 70% was used as a positive control, while samples incubated with medium alone were used as negative controls. Histograms showing live and dead cells as % of positive cells. All the experiments were performed in triplicate and shown as means ± SD (Two-Way ANOVA and student T Test). (b) PBMCs were treated with bismuthene (100 μg/mL) for 24 h or left untreated (Unt), LPS (2 μg/mL) and ConA (10 μg/mL, Sigma) were used as positive controls. Cell activation was evaluated by flow cytometry, PBMCs were gated by specific membrane markers for the two major subpopulations: T cells (CD3-FITC, Clone: UCHT1, BD) and monocytes (CD16 BV711, Clone 3G8, BD). Cells were stained with CD25-PE and CD69-FITC. Cell activation of PBMCs was expressed as % of positive cells. Histogram plots showing positivity for CD25-PE (Clone M-A251, BD) and CD69-BV421 (Clone FN50, BD) staining in PBMCs treated with bismuthene (100 μg/mL) Data are presented as mean ± SD of three independent samples. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (two-way ANOVA). (c) Cytokine production. IL-4, IL-5, IL-6, IL-10, IL-12, TNFα and IFNγ concentration measured in the supernatant of PBMCs treated with bismuthene (100 μg/mL) for 24 h, using BD Cytometric Bead Array(CBA) Kit.
Article Snippet: PBMCs were treated with different concentrations (10, 50, 100 and 200 μg/mL) of bismuthene for 24 h. Cell viability was analyzed by flow cytometry using
Techniques: Ex Vivo, Flow Cytometry, Staining, Positive Control, Incubation, Activation Assay, Membrane, Concentration Assay